Provide a PowerPoint presentation (approximately 5 to 8 slides) for a proposal of the sale of a seasonal item. Choose one item and include the following in your presentation:
SEASONAL ITEM: SWIMSUITS
Immunology Applications Project
Bio 152
For this project, you will be working on internet-based programs that will walk you through some of the practical applications of immunology. These immunological assays can be used to detect a wide variety of diseases and are regularly used in doctor’s offices and hospitals.
You can type your answers directly into this Word document; when you are done, print it out and turn it in on the day of the final exam. If you cut and paste from the website rather than putting things into your own words, you will not receive credit for the exercise.
Start out by going to the following YouTube video
What is an agglutination assay? What is the basis of the way in which this assay work
What types of antigens can be detected by an agglutination assay?
What types of tests are used clinically that are based on agglutination assay
Now, go to the following YouTube video to learn the basics about the ELISA. This will give you some background information for the next section of the exercise.
To get started on the next exercise, go to the following website:
https://media.hhmi.org/biointeractive/vlabs/immuno…
Once the website loads, you will see a page that has a picture on the left side of a laboratory, and a series of tabs on the right side that will allow you to work through the lab exercise.
First,
1.read the directions on the Diagnosis tab on the right side of the page
2.read the background information that is on the second tab
3.click on the Notebook tab.
You will work through this tab in conjunction with the interactive pictures on the left side of the page in order to complete the project.
What does the term ELISA mean?
Describe the four steps in the generalized ELISA protocol for detecting a target antigen.
Why must the patient’s whole blood samples be centrifuged at the start of the laboratory assay?
Why must you prepare three dilutions of the serum samples?
What does the acronym PBS mean?
Why have the wells of the ELISA plate been pre-treated with SLE antigen?
Why are both a positive and negative control used when running the test? What is used as the positive control? What is used as the negative control?
What is meant by the terms primary antibody and secondary antibody? How are they different from each other?
What does HRP stand for? What is its purpose in the test system?
What is the purpose of incubating the ELISA plate at 37 degrees C for 15 minutes? What will happen if the plate is not incubated properly?
What is the purpose of washing the wells in the plate with PBS after the incubation period?
What is the secondary antibody that is used in this experiment? How is it different from the primary antibody? What is the enzyme that is attached to this secondary antibody?
What is the purpose of the second incubation in the process? Why is it incubated at 37 degrees C?
What is the substrate for HRP in the test? How does the HRP work with the substrate to result in a yellow color if the test result is positive?
What are the results of the tests on the three patients? Who has the disease? Are the results of the test unequivocal in each test?
Print out the summary page of this exercise and attach it to this exercise when you turn it in.
Now, go to the following animation/ video to learn something about monoclonal antibody production.
http://www.sumanasinc.com/webcontent/animations/co…
What is a monoclonal antibody?
What is a hybridoma cell? Where does it come from? What is the purpose of this cell?
Raphael, Philosophy (School of Athens), Stanza della Segnatura, Vatican Palace, Rome, Italy, 1509 – 1511. Fresco, 19′ x 27′
500 word essay response.
Your answer should be at least 500 words and contain a thesis statement. Your essay response should use and define key terms that are relevant to and reference the content presented in this class.
The writing should be organized and free of any spelling or grammar issues.
In addition to using and defining key terms, referencing the content of this course, your essay should also be grounded in the form of the art itself. Be sure to offer visual descriptive examples found in the artwork as visual evidence to support your claims.
Do not do an internet search for this part of the exam. This is not a research assignment. The essay part of the exam is your opportunity to demonstrate that you have completed the assigned readings from the textbook and the modules. You will not receive credit for information not presented in this course. If your answer contains un-cited information from an outside source you will receive a “0†on the essay part of the exam.
Critical Thinking Assignment
“The increased use of technology by the police poses a significant threat to the civil liberties of the public.†Select a position on this debate and prepare an argument on behalf of your position. 500 word minimum. [LO 2,6,&7] Review the Critical Thinking Essay Rubric for grading guidelines.
………………………………………………………………………………
News Article
Each assignment will consist of a posting of a relevant news article or media or story relating to the topics for the week. The students will upload the topic with a link to the article or story and discuss the relevancy to the readings. 300 word minimum.
Review the News and Media Review Rubric
for grading guidelines.
This weeks objectives for reference to pick a topic:
Objectives
Upon completion of this week, you will be able to:
……………………………………………………………………………………………………
This is a Post-First Discussion. You must make your initial post prior to seeing the responses of your classmates.
The subject to be debated is “Has the Supreme Court of the 1980s, 1990s, and 2000s has swung too far to the right by overemphasizing the rights of society and underemphasizing individual rights?†Choose a side and cite as many cases as possible in the area of search and seizure in order to defend your stance. [LO 3 and 4]
Review the Discussion Scoring Rubric
for grading and participation guidelines.
I cannot see the discussion responses until the initial post is submitted. Due to this, can you either provide me with 2 general peer responses that I can post on whoever’s post or submit the initial post to me first so I can provide you the peers to respond to. Let me know when you are chosen as the tutor.
Pre Pare both questions in Good form, If any further questions feel free to ask.
Respond to the following in a minimum of 175 words:
Recall the readings and activities you have completed this week and research a TED Talk or other academic video that applies to one or more of your weekly objectives.
Objective: Technology based training methods
https://www.youtube.com/watch?v=AOTEQVYDPpg
Answer the following questions and include a link to the video:
Reply to at least two of your classmates. Be constructive and professional in your responses.
Student One
Today I watched a Ted Talks video with speaker Tony Wiggins titled Re-thinking the way we value older workers. Older workers this day in age are around 45 years or older. By the year 2020 4 out of 10 workers at the age of 45 or older will be out of work. Tony suggested if companies re-engaged worker 45 plus in age this would: create 625, 000 more workers, boost the economy, decrease homelessness, improve personal health and create support to farmers and regional towns. Employees forget that older workers something very vital to the company and that is workplace wisdom. They have well development communication, life experience, and resilience in turbulent times. I enjoyed this video because it relates to this week reading centered on training older workers. Although there may be a major change in how older workers adapt to changes in job demands, they have the ability to overcome because of their resilience.
Video viewed: https://www.youtube.com/watch?v=tVaeNy3QGec
Student Two
I chose a video this week involving a discussion on improving team performance. The video, “How to Turn a Group of Strangers into a Team,” by Amy Edmondson on TED talk, https://www.ted.com/talks/amy_edmondson_how_to_turn_a_group_of_strangers_into_a_team?utm_campaign=tedspread&utm_medium=referral&utm_source=tedcomshare, discusses what “Teaming” is and the formal definition of a team, and the difference between the two. Amy defines team as, “a stable, bounded group of individuals interdependent in achieving a shared goal.” She also defines “Teaming” as “teamwork on the fly” or the frequent changing of team members and how best to work together. The modern workforce is ever changing, so do the people doing the work. Everyone doesn’t get to choose their team members but working together is an integral part of the success of a business. Amy used the 2010 mine collapse in Chile as an example of her term “Teaming”. She went on to explain how different people from all over the world, different professionals, volunteers, and special forces came together to create a solution to recover the men trapped in the collapsed mine and rescue them as quickly and safely as possible.
define the two alternative concepts of Supreme Court jurisprudence: strict constructionism and judicial activism. Which political ideology is more associated with strict constructionism? Judicial activism? Provide one example of a U.S. Supreme Court ruling that adheres to strict construction and one that adheres to judicial activism. Which legal approach do you think is best?
the questions of 2 part :
part 1- talk about polices – 3-4 pages
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part 2- write paragraph for 8 topics
There will be a final paper of approximately 2000 words (about 6-8 pages), double spaced pages to be submitted on the day the paper is due on a topic to be chosen by the student. You should provide at least six peer reviewed sources to help substantiate points made throughout you essays. Just as with the Mini Essays, you must use Chicago Style for references. At the beginning of your essay, please write out fully which prompt you will be writing about, as sometimes it is not always clear for me as a reader.
You are required to choose one of the following prompts for your final essay.
• How has art influenced the development and understanding of Buddhism throughout history?
• Buddhism has adapted to many cultural and societal changes as it spread around the globe throughout history. How have modernity and globalization influenced the message and expansion of Buddhism within recent history?
• How are women perceived within the different schools of Buddhism? Have those perceptions changed over time? Why or why not?
• Buddhism has often found itself involved in violent actions, and have even been known to develop skills in martial arts. How is violence perceived in Buddhism? Do warrior monks or even Buddhist forms of terrorism fit within the spectrum of Buddhist teachings? Why or why not?
• What role does karma play in Buddhism? Who does it affect, and how does it affect them in this life, the afterlife, and the next life?
• According to the second noble truth, greed/craving give rise to suffering. What did the Buddha mean by greed/craving and how do they cause suffering? How do these apply to love and compassion for others?
What is the Lotus Sutra and why is it so important to Japanese Buddhism?
Your essays will be graded on the following criteria: a) evidence that the factual material called for in the question is presented and documented; b) coherence of the essay in organization and structure; c) ability to engage critically and analytically with the topic d) evidence of creative thinking; e) correct annotation of sources and presentation of bibliography; f) correct spelling, punctuation and grammar.
full page On each day, the main important things that happened in that day
with work cited after each day, so day 12 then cited work and then day 13 work cited
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