ie 3 lab report analysis of unknown insert mode 1

The assignment details have been attached below.

The lab notebook report for mode #1 analysis of your unknown insert should include: • The purpose of the experiment, all procedures used to isolate and prepare the vector DNA for cloning • An explanation of the expected results for BOTH the cat and kan insert positive clones that were supposed to be plated onto antibiotic plates. Discuss which plates would and wouldn’t show growth for each respective insert. • Any applicable graphs, tables, and photos must be included in your typed report and should be labeled so that they can be referenced in the conclusion of the report. • Your report must contain the following data: o Calculations used in determining concentrations of DNA and how much DNA needed to be added to ligation reactions o An overview of the transformation procedure and the logic you would have used for plating the cloned transformants onto specific antibiotic plates and the expected results for each insert.